Near infrared spectroscopy on-line and real-time monitoring of alcohol precipitation process of reduning injection / 中国中药杂志

Near infrared (NIR) spectroscopy as a kind of rapid process analysis technology has been successfully applied in Chinese medicine pharmaceutical process. In this research, the technology was adopted to establish the rapid quantitative analysis models of main indicators from the Lonicera japonica and Artemisia annua alcohol precipitation process of Reduning injection. On-line NIR spectra of 142 samples from alcohol precipitation process were collected and the content of main indicators for each sample were detected through off-line HPLC. With eliminating outliers, determination of spectra pretreatment method and selecting optimal band, the NIR quantitative calibration model for each indicator was established using partial least squares (PLS). These models were used to predict the unknown samples from precipitation process of Reduning injection to achieve the goal of rapid detection. The results showed that the models were ideal. The correlation coefficients of models for neochlorogenic acid, chlorogenic acid, 4-O-caffeoylquinic acid and secoxyloganin were 0.973 872, 0.985 449, 0.975 509 and 0.979 790, respectively and their relative standard errors of prediction (RSEP) were 2.922 49%, 2.341 37%, 2.930 40% and 2.184 60%, respectively. This study indicated that the NIR quantitative calibration model showed good stability and precision, and it can be used in rapid quantitative detection of main indicators of efficacy in order to on-line monitor the alcohol precipitation process of Reduning injection.

Application of hypericin in cancer therapy and diagnosis / 国际药学研究杂志

Hypericin, used as a medicinal heifo since ancient times, is a natural photosensitizer derived from the plant Hypericum perforantum. Hypericin would produce peroxides to induce apoptosis and inhibit the growth of oncocytes. Meanwhile, hypericin has a specific affinity with tumor pathology organization (underlying accumulation in pathology organization) which has been widely used in optical diagnosis. The present review gives a comprehensive summary of the anticancer effect based on photodynamic therapy, and photodynamic diagnosis of hypericin optical properties, with the purpose of making full use of the hypericin natural resources, and providing a basis for research and development of hypericin derivatives. © 2006 Editorial office of Foreign Medical Sciences.